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Cloning 101: Introduction to Technology, Target Customers, Applications (CLON-101)


Description
Upon completion of this course, the learner will be able to:
+ explain what plasmid cloning is, who does it, and why it is done
+ identify the most common methods of plasmid cloning
+ describe critical components and mechanics of plasmid cloning
+ articulate common applications of cloning
+ explain basic choices made by cloning users, depending on the application
The learner will view videos, read articles, and answer questions before completing a quiz to check for complete comprehension.
Learners are encouraged to bring their own knowledge, use search engines, and ask questions (marcia_mednick@takarabio.com) throughout their learning journey.
Content
  • Cloning 101.1: Introduction to Plasmids and Bacterial Transformation
  • Cloning 101.2: Plasmid Cloning by Restriction Digest and Ligation
  • Cloning 101.3: Plasmid Cloning by Topoisomerase I
  • Cloning 101.4: Plasmid Cloning by Gibson Assembly
  • Cloning 101.5: Plasmid Cloning by Gateway
  • Cloning 101.6: Plasmid Cloning by Golden Gate Assembly
  • Cloning 101.7: Plasmid Cloning with In-Fusion SNAP
  • Cloning 101.8: Cloning Workflows
  • Cloning 101.9: Plasmid Cloning Quiz
Completion rules
  • All units must be completed